Image Quality: Resolution, Contrast and the MTFMatching Optical Resolution with Pixel Resolution

40x Lens

NA = 0.76

0.8 um

A Pixel

subtends

0.4 um

8 um

pixels

Both the lens and the CCD can

resolve the objects

The optics and the CCD are “matched”.

Optical Resolution:

D = 0.61 x (wavelength) / Numerical Aperture

D = 0.61 x 0.5 / .7 = 0.4 um

Pixel Resolution:

P = Pixel Size x 2 / Magnification

P = 8 x 2 / 40 = 0.4 um

psf

Image Quality: Resolution, Contrast and the MTF Matching Optical Resolution with Pixel Resolution